Fig 2.

Effects of iap titration on OSA levels in various wzz knockout strains. Shown are Western immunoblot assays of LPS prepared from P. aeruginosa PAO1 wzz chromosomal mutants transformed with pHERD20T-His₆-Iap. Expression levels were titrated via increasing concentrations of l-arabinose (0, 0.1, 0.5, and 1.0%). Immunoblot assays were subsequently probed with MAbs specific to O5 OSA (MAb MF15-4) and inner core oligosaccharide (MAb 5c-7-4) (*). WT P. aeruginosa PAO1 and PAO1 transformed with pUCP26iap were used as WT and OSA⁻ controls in each set of blots. (A) LPS harvested from the wzz₁ chromosomal mutant background. (B) LPS harvested from the wzz₂ chromosomal mutant background. (C) LPS harvested from the wzz₁ wzz₂ double chromosomal mutant.