Sequence alignment of Iap with Wzz1 (A) and Wzz2 (B) of P. aeruginosa PAO1. The alignment was produced in ClustalW and colored according to conservation score (out of 10) as represented by Jalview, where red is 10, orange is 9, yellow is 8, and blue is 7. The black line denotes the predicted transmembrane region of either Wzz protein. Both alignments demonstrate strong similarities between the Iap sequence and Wzz from P. aeruginosa strain PAO1, alluding to substrate specificity.