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Topology prediction algorithm analysis of Iap. The amino acid sequence of Iap was analyzed by nine different algorithms to determine whether a conserved single TMS-spanning domain is predicted for a putative TMS. The sequence of Iap amino acids 1 to 31 is depicted. Color key: blue line, cytoplasmic localization; black box, range of amino acids predicted to form a TMS; red line, periplasmic localization.
