Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype, phenotype, or relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| P. aeruginosa | ||
| O5 | Wild-type strain PAO1, IATS O5, A+ B+ | 61 |
| O16 | Wild-type strain, IATS serotype O16, A− B+ | ATCC 33363 |
| wzz1::Gmr | PAO1 derivative, A+ B+a | 12 |
| wzz2Δ | PAO1 derivative, A+ B+ | 11 |
| wzz2Δ wzz1::Gmr | PAO1 derivative, A+ B+ | 11 |
| wzyβ::Gmr | O16 derivative, A− B− | 28 |
| E. coli DH10B | F− araD139 Δ(ara leu)7697 ΔlacX74 galU galK rpsL deoR ϕ80dlacZΔM15 endA1 nupG recA1 mcrA Δ(mrr hsdRMS mcrBC) | Laboratory stock |
| Plasmids | ||
| pHERD20t | Pseudomonas expression vector (Plac); Apr | 29 |
| pHERD20tHis6iap | pHERD20t carrying His6-iap | This study |
| pET28His6iap | Escherichia expression vector | 27 |
| pUCP26 | 4.9-kb pUC18-based broad-host-range vector, Tcr | 43 |
| pUCP26iap | Broad-host-range vector containing iap | 27 |
| pUCP26wzz1 | Broad-host-range vector containing O5 wzz1 | 12 |
a
A superscript plus or minus after A or B denotes the presence or absence of the particular O polysaccharide, respectively. Resistance (superscript r) is shown for gentamicin (Gm), ampicillin (Ap), and tetracycline (Tc).