Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| E. faecalis | ||
| OG1RF | Wild-type strain, Fusr Rifr p-Cl-Pher | 32 |
| OG1RF Tn library | OG1RF Tn insertion mutants | 13 |
| CK111 | Conjugative donor | 12 |
| ΔccpA mutant | Deletion of ccpA from OG1RF | This study |
| pMSP/ccpA::Tn917 strain | ccpA::Tn917-containing plasmid pMSP3535-tet | This study |
| pMSP-ccpA/ccpA::Tn917 strain | ccpA::Tn917-containing plasmid pMSP-ccpA | This study |
| TX5467 | OG1RF ace (Δace) deletion mutant | 6 |
| JH2-2 | ||
| CL14 | JH2-2 ccpA insertion mutant | 33 |
| E. coli | ||
| XL1-Blue | Cloning host | Stratagene |
| EC1000 | Host for pCJK47 | 12 |
| Plasmids | ||
| pGEM-T Easy | Cloning plasmid | Promega |
| pMSP3535 | Nisin-inducible expression vector, Emr | 34 |
| pMSP3535-tet | Nisin-inducible expression vector | This study |
| pMSP-ccpA | pMSP3535-tet carrying a ccpA fragment encoding residues 1–333 | This study |
| pCJK47 | Plasmid for markerless exchange | 12 |
| pMC1 | Deletion of ccpA allele in pCJK47 | This study |