Fig 3.

Inhibition of TagO with tunicamycin does not suppress the growth defects of the Δlcp mutant. (A) Bacterial growth was examined by plating serial dilutions of cultures grown for 3.5 h without or with tunicamycin (0 or 1 μg/ml). Culture aliquots of the wild type and the Δlcp mutant without or with plcpA, plcpB, or plcpC were spotted on agar plates. A₆₀₀ values were recorded at the time of plating and are reported under the images of the agar plates. (B) Membrane integrity of staphylococci assessed with propidium iodide staining. Culture aliquots of the wild type and the Δlcp mutant grown for 3.5 h without or with tunicamycin (0 or 1 μg/ml) were fixed and stained as described in the legend to Fig. 2B. The data were analyzed as described in the legend to Fig. 2B. *, P < 0.05). (C) Transmission electron micrographs of wild-type and Δlcp mutant cells from cultures grown for 3.5 h without or with tunicamycin (0 or 1 μg/ml). Bars, 200 nm.