Table 1.

Strains and plasmids used in this study

Strain or plasmid	Genotype or insert	Description	Reference or source
Strains
RN4220	Wild type	S. aureus laboratory strain, a restriction-deficient derivative of NCTC 8325-4	56
RN4220 ΔtagO	RN4220 tagO	RN4220 lacking tagO	57
RN4220 tagB::erm	RN4220 tagB	RN4220 with a bursa aurealis insertion in tagB	This work
RN4220 tarF::erm	RN4220 tarF	RN4220 with a bursa aurealis insertion in tarF	This work
RN4220 tarJ2::erm	RN4220 tarJ2	RN4220 with a bursa aurealis insertion in tarJ2	This work
MSSA 1112	Wild type	MSSA clinical isolate	58
MSSA 1112 ΔlcpA	MSSA 1112 lcpA::erm	MSSA 1112 with ermB replacing lcpA (msrR)	46
MSSA 1112 ΔlcpB	MSSA 1112 lcpB	MSSA 1112 lacking lcpB (sa0908)	33
MSSA 1112 ΔlcpC	MSSA 1112 lcpC	MSSA 1112 lacking lcpC (sa2103)	33
MSSA 1112 ΔlcpAB	MSSA 1112 lcpA lcpB	MSSA 1112 lacking lcpA and lcpB	33
MSSA 1112 ΔlcpAC	MSSA 1112 lcpA lcpC	MSSA 1112 lacking lcpA and lcpC	33
MSSA 1112 ΔlcpBC	MSSA 1112 lcpB lcpC	MSSA 1112 lacking lcpB and lcpC	33
MSSA 1112 Δlcp	MSSA 1112 lcp	MSSA 1112 lacking all three lcp genes	33
MSSA 1112 Δlcp ΔtagO	MSSA 1112 lcp tagO	MSSA 1112 lacking all three lcp genes and tagO	This work
Plasmids
pΔtagO	DNA segments flanking tagO	pKOR1 carrying a 1-kbp DNA segment upstream and downstream of tagO, used for allelic replacement	This work
pHTT4	SEB-MH6-CWS	pOS1 vector encoding SEB with the CWS motif of protein A	10
pBursa	Modified mariner transposon	Plasmid carrying the mariner-based transposon with erythromycin resistance (thermosensitive replicon)	36
pFA545	Mariner transposase	Plasmid carrying the transposase (thermosensitive replicon)	36
plcpA	lcpA (msrR)	Plasmid pGC2 (pT194 based) encoding lcpA for complementation studies	33
plcpB	lcpB (sa0908)	Plasmid pGC2 (pT194 based) encoding lcpB for complementation studies	33
plcpC	lcpC (sa2103)	Plasmid pGC2 (pT194 based) encoding lcpC for complementation studies	33