Table 2.
Sortase A-catalyzed surface protein anchoring to the cell wall is not altered in the Δlcp mutanta
| Ion | Observed m/z |
Calculated m/z | Proposed structure | |
|---|---|---|---|---|
| Wild type/pHTT4 | Δlcp/pHTT4 | |||
| 1 | 1,665.941 | 1,665.811 | 1,665.729 | NH2-H6AQALPET-Gly2-CO2H |
| 2 | 1,693.893 | 1,693.830 | 1,693.739 | HCO-NH-H6AQALPET-Gly2-CO2H |
| 3 | 1,722.888 | 1,722.827 | 1,722.781 | NH2-H6AQALPET-Gly3-CO2H |
| 4 | 1,750.956 | 1,750.867 | 1,750.791 | HCO-NH-H6AQALPET-Gly3-CO2H |
| 5 | 3,826.572 | 3,826.436 | 3,826.215 | NH2-VDSKDVKIEVYLTTKKGTMH6AQALPET-Gly2-CO2H, Na+ |
| 6 | 3,854.116 | 3,854.111 | 3,854.225 | HCO-NH-VDSKDVKIEVYLTTKKGTMH6AQALPET-Gly2-CO2H, Na+ |
| 7 | 3,901.934 | 3,902.469 | 3,903.301 | H2NCO-NH-VDSKDVKIEVYLTTKKGTMH6AQALPET-Gly3-CO2H |
a
Cyanogen bromide cleavage products of cell wall-anchored SEB-MH6-CWS liberated by lysostaphin treatment and purified by Ni-NTA chromatography (Fig. 4) were analyzed by MALDI-TOF MS in linear positive mode. The program CS ChemDraw was used for m/z calculations.