Fig 4.

Effects of hydroxylamine-induced (H) and site-directed (S) nanR mutations in a nanA′ reporter, nanR⁺ background. The indicated plasmids expressing wild-type or mutant nanR were induced with arabinose, and extracts were subjected to Western blot analysis against anti-NanR antibody, as indicated by the expected 30-kDa bands shown in lane 1 and lanes 3 through 10. Lane 2 is a positive control containing 0.2 μg of purified NanR as the antigen. Amino acid changes in the mutated NanR are indicated. Effects of the unmutagenized and mutant NanR expressed by the plasmids on reporter fusion activity are indicated by the average activities obtained from three separate experiments, with the SD in parentheses.