Fig 7.

Nan boxes in E. coli and selected bacteria predicted to encode orthologs of NanR. (A) One strand of the 49-bp oligonucleotide used for modified Ferguson analysis is depicted, with boxed nucleotides indicating the conserved regions in the nanATEK-yhcH, nanCMS, and yjhBC operators (Fig. 1E). Nucleotides above the box indicate overlapping repeats of the GntR superfamily consensus sequence, where lowercase letters signify deviations from the consensus and “n” denotes any nucleotide. Brackets indicate nucleotides deviating from the Nan box sequence. Nucleotides below the box indicate the overlapping FadR consensus sequence (41). Underlined nucleotides indicate the hexanucleotide repeats typically found in Nan boxes. (B) Nan boxes in bacteria with NanR orthologs. Sd, Shigella dysenteriae; Ck, Citrobacter koseri; St, Salmonella enterica serovar Typhimurium; Es, Enterobacter sakazakii; Et, Edwardsiella tarda; Sp, Shewanella pealeana; Ph, Pseudoalteromonas haloplanktis; Cf, Citrobacter freundii.