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Suppressor mutations increase SecY protein levels relative to the secA2 K129R strain. Whole-cell lysates and subcellular fractions were separated by SDS-PAGE, and SecY protein was detected by Western blotting. All samples were equally loaded. Strains tested were the following: complemented secA2 (NR172), ΔsecA2 + empty plasmid (NR160), secA2 K129R (NR178), 4S (NR236), and 24S (NR151).
