Table 2.

Plasmids used in this study

Plasmid	Genotype	Description	Source
pMV306.kan	aph int attPL5 ColE1	Single-copy mycobacterial shuttle vector, integrates in mycobacteriophage L5 attB site	39
pMV361.kan	aph Phsp60 int attPL5 ColE1	Single-copy mycobacterial shuttle vector with hsp60 promoter, integrates in mycobacteriophage L5 attB site	39
pYA810	aph Phsp60-secA2 int attPL5 ColE1	M. smegmatis secA2 in pMV361.kan	15
pNR25	aph Phsp60-secA2K129R int attPL5 ColE1	M. smegmatis secA2 K129R in pMV361.kan	17
pCR2.1-TOPO	aph bla ColE1	TOPO TA cloning plasmid	Invitrogen
pLL5	aph bla PsecY-secY′ ColE1	M. smegmatis secY promoter and 34 bp of secY gene in pCR2.1-TOPO	This work
pLL6	aph bla PsecY(4S)-secY′ ColE1	NR236 (4S) secY promoter and 34 bp of secY gene in pCR2.1-TOPO	This work
pCV125	hyg ′lacZ oriM ColE1	Multicopy mycobacterial shuttle vector with promoterless lacZ gene	MedImmune
pLL11	hyg PsecY-secY′-′lacZ oriM ColE1	M. smegmatis secY translationally fused to lacZ in pCV125	This work
pLL8	hyg PsecY(4S)-secY′-′lacZ oriM ColE1	NR236 (4S) secY translationally fused to lacZ in pCV125	This work
pLL15	hyg PsecY(24S)-secY′-′lacZ oriM ColE1	NR151 (24S) secY translationally fused to lacZ in pCV125	This work
pLL17	aph bla secY ColE1	M. smegmatis secY in pCR2.1-TOPO	This work
pLL19	aph bla secY(4S) ColE1	NR236 (4S) secY in pCR2.1-TOPO	This work
pYUB2063	hyg bla int attPTweety ColE1 cosλ	Single-copy mycobacterial shuttle vector, integrates in mycobacteriophage Tweety attB site	W. R. Jacobs, Albert Einstein College of Medicine
pLL2	hyg bla int attPTweety ColE1	pYUB2063 with 3,299-bp PciI fragment removed, single-copy mycobacterial shuttle vector, integrates in mycobacteriophage Tweety attB site	This work
pLL21	hyg bla secY int attPTweety ColE1	M. smegmatis secY in pLL2	This work
pLL23	hyg bla secY(4S) int attPTweety ColE1	NR236 (4S) secY in pLL2	This work