Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| S. aureus strains | ||
| Newman | Wild-type CP5 strain | T. Foster |
| CYL6841 | Newman ΔclpC::cat | 14 |
| CYL12447 | Newman ΔclpC::cat(pJG4017) | This study |
| CYL12448 | Newman ΔclpC::cat(pLL31) | This study |
| CYL12683 | Newman ΔclpC::cat(pJG4080) | This study |
| CYL6620 | Newman ΔsbcC | 15 |
| IK184 | Newman ΔrsbUVWsigB | 16 |
| NE1922 | USA300 nusG::bursa | NARSA |
| NE1495 | USA300 murA::bursa | NARSA |
| NE460 | USA300 atl::bursa | NARSA |
| E. coli strains | ||
| DH5α | Host strain for plasmids | Invitrogen |
| XL1 Blue | Host strain for plasmids | Stratagene |
| BTH101 | Recipient strain for two-hybrid system | 17 |
| Plasmids | ||
| pLL31 | E. coli-S. aureus shuttle vector with Pspac | 14 |
| pJG4017 | pLL31-clpCtrap-His6 | This study |
| pJG4080 | pLL31-clpC-His6 | This study |
| pKT25 | Bacterial two-hybrid system vector | 17 |
| pUT18C | Bacterial two-hybrid system vector | 17 |