Table 2.
Primers used in this study
| Primer use and name | Sequencea |
|---|---|
| Primers for ClpCtrap construction | |
| ClpCtrap1 | 5′-GAATTCTGGAAGAGGCTTCTTATAGAT-3′ |
| ClpCtrap2 | 5′-AGTATGCAACGCATCAATAAATAG-3′ |
| ClpCtrap3 | 5′-CTATTTATTGATGCGTTGCATACT-3′ |
| ClpCtrap4 | 5′-GGTGTCGTATGACTCTTAAGTCTTAC-3′ |
| ClpCtrap5 | 5′-GTAAGACTTAAGAGTCATACGACACC-3′ |
| ClpCtrap9 | 5′-TCAATTGCATCAAATAAAATTACAGAATATGGTTTAC-3′ |
| ClpCtrap10 | 5′-CATATTCTGTAATTTTATTTGATGCAATTGAAAAAGCTCAT-3′ |
| ClpCtrap8 | 5′-GGATCCatggtgatggtgatgatgTCCACCTGCTTGCGATGGTGTTTTAGTTTC-3′ |
| ClpCtrap11 | 5′-GGATCCatggtgatggtgatgatgTCCCCATGCTTGCGATGGTGTTTTAGTTTC-3′ |
| Primers for two-hybrid test | |
| 2h clpC F | 5′-CTGCAGTCATTATTTATGTTATTTGGTAGATT-3′ |
| 2h clpC R | 5′-GGATCCTGCTTGCGATGGTGTTTTAGTTTC-3′ |
| 2h ctsR F | 5′-CTGCAGGTGATATACATGCACAATATGTCT-3′ |
| 2h ctsR R | 5′-GGATCCGTAATAATTTATAACTGGTAACAA-3′ |
| 2h mecA F | 5′-CTGCAGTGAGATGATATGAGAATAGAA-3′ |
| 2h mecA R | 5′-GGATCCTTCAGTTGTCTCTGGAAAATA-3′ |
| 2h codY Fnew | 5′-CTGCAGGAAAAATTCATGAGCTTATTATCT-3′ |
| 2h codY Rnew | 5′-GGATCCTTTACTTTTTTCTAATTCATCTAA-3′ |
| 2h rsbW Fnew | 5′-CTGCAGTCGAATAACATGCAATCTAAAGAA-3′ |
| 2h rsbW Rnew | 5′-GGATCCGCTGATTTCGACTCTTTCGCCATT-3′ |
a
Underlined sequences denote restriction sites. Nucleotides shown in bold are mutations made for clpCTrap construction. Lowercase letters denote the His6 sequence.