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Growth curves of the conditional acpS mutant in liquid LB media. Cultures of IacP_TAP and PBADacpS IacP_TAP were grown for 3 h in LB to expand for about 5 generations of growth. Cultures then were diluted to an OD600 of 0.05 in LB-0.1% arabinose, LB-0.1% glucose, or LB, and the optical density was monitored. Cultures were incubated at 37°C with shaking.
