Fig 3.
CS448-3 exhibits wild-type migration and an attenuated Rcs response after 24 h of incubation. (A) Strains were assayed for migration at 10 and 24 h. At 10 h, CS109 migrates (top left), but CS448-3 does not migrate (bottom left). CS448-3 recovers wild-type migration after 24 h (bottom right, white dotted circle indicates migration ring). (B) Cells were removed from the migration plate and assayed for PrprA::sfgfp expression (black bars, 10 h; gray bars, 24 h). Each strain contained plasmid pDKR1, which contains the PrprA::sfgfp reporter. Reporter expression in CS448-3 pDKR1 returns to wild-type levels at 24 h. “+”, Wild type rcsB is present; “−”, rcsB is deleted. The data from three experiments were normalized relative to CS109.