Fig 4.
The Cpx stress response is activated in E. coli CS448-3. (A) Migration assays: A1, CS109; A2, CS448-3; A3, CS109 ΔflhA (SKCS49-1). (B) Deleting cpxR restores the ability to migrate to CS448-3: B4, CS109 ΔcpxR (KE36); B5, CS448 ΔcpxR (KE37). (C) Cpx reporter activity is increased in CS448-3 and is independent of the Rcs phosphorelay. (D) Cpx reporter activity in double and triple PBP mutants. Strains are the same as in Fig. 2D. (E) Rcs reporter activity depends on CpxR. The experimental details are the same as in the legend to Fig. 2. The strains in panels C and D contain plasmid pJW1, which carries the PcpxP::luxCDABE reporter. The strains in panel E contain plasmid pDKR1, which carries the PrprA::sfgfp reporter. “+”, rcsC or cpxR are present; “−”, the gene is deleted. In panels C, D, and E, the data from three experiments were normalized relative to CS109.