Table 1.
E. coli strains and plasmids
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| E. coli strains | ||
| CS109 | W1485 rph-1 katF (rpoS) | 3 |
| CS204-1 | CS109 dacA::res pbpG::res | 3 |
| CS206-3 | CS109 ampH::res pbpG::res | 3 |
| CS315-1 | CS109 dacA::res pbpG::res dacB::res | 3 |
| CS317-3 | CS109 ampH::res pbpG::res dacB::res | 3 |
| CS326-3 | CS109 ampH::res dacA::res dacB::res | 3 |
| CS345-3 | CS109 ampH::res dacA::res pbpG::res | 3 |
| CS448-3 | CS109 dacB::res dacA::res pbpG::res ampH::res | 3 |
| KE2 | CS109 ampG::kan | This study |
| KE8 | CS109 rcsB::tet | This study |
| KE9 | CS448-3 rcsB::tet | This study |
| KE11 | CS109 rcsF::cam | This study |
| KE12 | CS448-3 rcsF::cam | This study |
| KE14 | CS109 rcsC52::tet | This study |
| KE15 | CS448-3 rcsC52::tet | This study |
| KE18 | CS109 envZ::kan | This study |
| KE19 | CS109 ompR::kan | This study |
| KE20 | CS448-3 envZ::kan | This study |
| KE21 | CS448-3 ompR::kan | This study |
| KE36 | CS109 cpxR::kan | This study |
| KE37 | CS448-3 cpxR::kan | This study |
| KE39 | CS448-3 ampG::kan | This study |
| KE41 | CS109 fliC::kan | This study |
| KE42 | CS448-3 fliC::kan | This study |
| SKCS49-1 | CS109 flhA::kan | This study |
| Plasmids | ||
| pCP20 | cI857 repA(Ts) PR::flp, Ampr Camr | 25 |
| pDKR1 | colE1 PrprA::sfgfp; Kanr | 52 |
| pFlhDC | PBAD-flhDC; Camr | R. Harshey |
| pJW1 | pNLP10 with PcpxP::luxCDABE; Kanr | 33 |
| pKD46 | Lambda Red+ (Ampr pSC101 oriTS) | 25 |
| pLCM1 | Plac-ampH lacIq; Kanr | 53 |
| pLP8 | Plac lacIq, Kanr, pMLB1113 background | 54 |
| pLP405 | PBAD-dacB(S62A); Kanr | P. Potluri |
| pLP514 | Plac-dacA(S44G) lacIq; Kanr | 54 |
| pLP515 | Plac-dacA lacIq; Kanr | 54 |
| pLP706 | PBAD-pbpG(S67A); Kanr | P. Potluri |
| pLP18 | PBAD, Kanr | 55 |
| pLPKC403 | PBAD-dacB, Kanr | 55 |
| pLPKC704 | PBAD-pbpG, Kanr | 55 |
| pMEL1 | Plac-ampH(S87A) lacIq; Kanr | 53 |
| pVS182 | flhD::lacZ in pRS551; Ampr | 56 |
a
Camr, chloramphenicol resistance; Ampr, ampicillin resistance; Kanr, kanamycin resistance.