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Detection of gene expression levels of aveR, aveA1, and SAV7472-SAV7473 in D7471 (the SAV7471 deletion mutant) and O7471 (SAV7471 overexpression transformant) by RT-PCR. RNA was prepared from cells grown in fermentation medium for 2 days and 6 days at 28°C with reciprocal shaking (220 rpm). hrdB (SAV2444), encoding the principal sigma factor, was used as the internal control. Control experiments with no reverse transcriptase confirmed that the RNA samples contained no chromosomal DNA.
