Fig 4.

DNase I footprinting of the coding strand of the SAV7472 promoter region identified using His₆-SAV7471. (A) The fluorograms correspond to the control DNA (with 10 μM bovine serum albumin [BSA]) and to the protection reactions (with 2 μM and 8 μM His₆-SAV7471). Site 1 and site 2 indicate the corresponding sites protected by SAV7471. The arrows indicate the characteristic inverted repeat sequences. (B) Analyses of the consensus sequence of SAV7471 binding sites. S = G or C; W = A or T. The arrows indicate the characteristic inverted repeat sequences. (C) Nucleotide sequences of the promoter regions of SAV7471 and SAV7472. Sequences protected from DNase I digestion are indicated with shaded boxes (site 1 and site 2). The transcriptional start sites are indicated by dark bent arrows and boldface letters. The presumptive −35 and −10 elements of SAV7471 and SAV7472 promoters are boxed. The translational start codons are indicated by gray bent arrows and boldface letters.