Fig 2.
Phenotype of the all1549Ωsp/sm mutant. (A) Genomic context of relana from Anabaena. The black triangle indicates the position of disruption following single-crossover recombination. (B) Growth on solid agar plates with nitrogen (NO3−) or without nitrogen (N2). (C) Micrographs of the wild type (WT), the all1549Ωsp/sm mutant, and the complemented strain (C1549) after nitrogen deprivation. The arrowheads point to heterocysts. (D and E) Measurement of growth rate. Strains were grown in BG11 (+N) (D) or in BG110 (−N) (E). Error bars indicate standard deviations of the results determined from triplicate cultures.