Fig 3.
Spatiotemporal expression of relana. (A) Transcriptional levels of relana gene in wild type (WT). The result determined with the hetR gene was used as the positive control. The rnpB gene was selected as the reference gene for normalizing the data obtained by qPCR. (B) Localization of full-length and truncated Relana in the wild-type strain bearing plasmids pRL25N-1549-lgfp and pRL25N-ACT-lgfp, respectively. P1549 represents the native promoter. The results were visualized under bright-field and fluorescence microscopy at 24 h after nitrogen starvation. Arrowheads indicate heterocysts.