Table 1.
Strains and plasmids
| Strain or plasmid | Descriptiona | Source |
|---|---|---|
| Strains | ||
| Anabaena sp. PCC 7120 | Wild type | Pasteur Culture Collection |
| Anabaena all1549Ωsp/sm | Disruption relana; Spr Smr | This study |
| Anabaena C1549 | Nmr Spr Smr; pRL25T-C1549 introduced into all1549Ωsp/sm strain | This study |
| E. coli MG1655 | Wild-type K-12 strain | 37 |
| E. coli CF1693 | MG1655 ΔrelA251 ΔspoT207 | 37 |
| Plasmids | ||
| pGEX-6p-1 | Expressing vector | GE Healthcare |
| pGEX-6p-1549 | pGEX-6p-1 carrying relana ORF | This study |
| pRL277 | Smr Spr; sacB-bearing cloning vector | 33 |
| pRL277-HRS | Smr Spr; pRL277 carrying an internal fragment (from position 277 to 1173 relative to the translational initiation codon) of the relana gene | This study |
| pRL25T | Kmr Nmr; pDU1-based shuttle vector | 32, 45 |
| pRL25T-p69-gfp | Kmr Nmr; pRL25T carrying relana promoter and gfp coding sequence | This study |
| pRL25T-C1549 | Kmr Nmr; pRL25T containing relana ORF and native promoter, used to complement mutant | This study |
| pRL25T-PpetE-hetR | Kmr Nmr; pRL25T carrying PpetE-hetR fusion | This study |
| pRL25N-1549-lgfp | Kmr Nmr; pRL25N-lgfp carrying relana under the control of native promoter | This study |
| pRL25N-ACT-lgfp | Kmr Nmr; pRL25N-lgfp carrying relana without ACT domain coding sequence under the control of native promoter | This study |
a
Km, kanamycin; Nm, neomycin; Sm, streptomycin; Sp, spectinomycin.