Table 1.
Bacterial strains and plasmids used
| Strain or plasmid | Genotype or descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| XL10 | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F′ proAB lac IqZΔM15 Tn10(Tetr) Amy Cmr] | Stratagene |
| BL21-CodonPlus(DE3)-RIL | F− ompT hsdS(rB− mB−) dcm+ Tetr gal λ (DE3) endA Hte [argU ileY leuW Cmr] | Stratagene |
| EC1000 | F− RepA+ araD139 (araABC-leu)7679 galU galK lacX74 rspL thi Kmr | 61 |
| BL21 Star-pLysS | F− omp T hsdSB(rB− mB−) gal dcm rne 131 (DE3) pLysS (Camr) | Invitrogen |
| Top10 | F− mcr A Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| DH5α | F− ϕ80lacZΔM15 Δ(lacZ YA-argF)U169 recA1 endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1 λ− | Invitrogen |
| One Shot Mach1-T1R | F− ϕ80(lacZ)ΔM15 Δlac X74 hsdR(rK− mK+) ΔrecA1398 endA1 tonA | Invitrogen |
| E. faecalis | ||
| JH2-2 | Rifr Fusr | 62 |
| OG1RF | Rifr Fusr | 63 |
| CK111 | OG1Sp upp4::P23repA4 Specr | 39 |
| OG1X | Smr | 64 |
| Plasmids | ||
| pIP501 | tra+ Cmr MLSr | 65 |
| pMAL-c2x | Ptac lacIq malE lacZ Apr | New England Biolabs |
| pQTEV | Pt4 lacIq His7 Apr | 38 |
| pBlueScript SK− | pUC ori lacZ Apr | Stratagene |
| pCJK47 | oriTpCF10 P-pheS* pORI280 derivative Emr | 39 |
| pCF10-101 | pCF10ΔoriT2 | 39 |
| pKA | pCJK47 aacA-aphD at BglII site; Emr Gentr | This study |
| pKAΔtraG | pKA with traG up- and downstream regions at EcoRI/XbaI sites | This study |
| pIP501ΔtraG | pIP501 traG in-frame deletion | This study |
| pEU327 | E. coli/G+ bacterial shuttle plasmid, Specr xylA promoter | 42 |
| pEU327-traGΔTMH | pEU327 with traGΔTMH | This study |
a
Tetr, tetracycline resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Rifr, rifampin resistance; Fusr, fusidic acid resistance; Specr, spectinomycin resistance; Smr, streptomycin resistance; Apr, ampicillin resistance; Gentr, gentamicin resistance; MLSr, macrolide-lincosamide-streptogramin B resistance; tra+, transfer proficient.