Fig 3.

Localization of GFP-PomB variants coexpressed with wild-type PomA in a fliG deletion strain of V. cholerae. Cells were grown aerobically in liquid medium containing 0.4 mM (left column) or 10 mM (right column) arabinose. After 5 h, 20 μl culture was spotted on poly-l-lysine-covered glass slides and the slides were inspected using a fluorescence microscope to observe GFP-PomB (left pictures in each panel). The same section of the microscopic preparation was also observed in the DIC mode of the microscope (right pictures in each panel). Bars, 5 μm.