Table 1.
Plasmids
| Plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| pEC422 | Confers His6 tag to the N terminus of a target protein, Apr | 47 |
| pISC-2 | Para araC Apr | 48 |
| pET24b | PT7, confers His6 tag to the C terminus of a target protein, Kanr | Novagen |
| pET224-Streptag | PT7, confers Strep tag to the C-terminus of a target protein, Kanr | 49 |
| pISC-H | MCS of pISC-2 inserted into pEC422 | 13 |
| pAB | ParaBAD, codes for His6-PomA and PomB-Strep, Apr | 13 |
| pAB-D23N | ParaBAD, codes for His6-PomA and D23N variant of PomB-Strep, Apr | 13 |
| pAB-D23E | ParaBAD, codes for His6-PomA and D23E variant of PomB-Strep, Apr | 13 |
| pAB-S26A | ParaBAD, codes for His6-PomA and S26A variant of PomB-Strep, Apr | This work |
| pAB-S26T | ParaBAD, codes for His6-PomA and S26T variant of PomB-Strep, Apr | This work |
| pAB-D42N | ParaBAD, codes for His6-PomA and D42N variant of PomB-Strep, Apr | This work |
| pAB-NheI-AgeI | ParaBAD, codes for His6-PomA and PomB-Strep, NheI and AgeI restriction sites upstream of pomB, Apr | This work |
| pABGFP | ParaBAD, codes for His6-PomA and GFP-PomB-Strep, Apr | This work |
| pABGFP-D23N | ParaBAD, codes for His6-PomA and D23N variant of GFP-PomB-Strep, Apr | This work |
| pABGFP-D23E | ParaBAD, codes for His6-PomA and D23E variant of GFP-PomB-Strep, Apr | This work |
| pABGFP-S26A | ParaBAD, codes for His6-PomA and S26A variant of GFP-PomB-Strep, Apr | This work |
| pABGFP-S26T | ParaBAD, codes for His6-PomA and S26T variant of GFP-PomB-Strep, Apr | This work |
| pET18ABS | PT7, codes for PomA and PomB-Strep, Kanr | 13 |
| pET18ABS-D23N | PT7, codes for PomA and D23N variant of PomB-Strep, Kanr | This work |