Table 2.
Cellular localization of GFP-PomB in V. choleraea
| V. cholerae strain | No. (%) of cells with GFP fluorescence signal |
|||
|---|---|---|---|---|
| At one pole | At two poles | Membrane associated | Total | |
| GFP-PomB (wt) | 197 (39.4) | 222 (44.4) | 81 (16.2) | 500 (100) |
| GFP-PomB-D23E | 128 (39.8) | 184 (57.1) | 10 (3.1) | 322 (100) |
| GFP-PomB-D23N | 101 (29.5) | 225 (65.8) | 16 (4.7) | 342 (100) |
| GFP-PomB-S26A | 232 (46.4) | 161 (32.2) | 107 (21.4) | 500 (100) |
| GFP-PomB-S26T | 259 (51.8) | 191 (38.2) | 50 (10.0) | 500 (100) |
| GFP-PomB-D42N | 117 (38.4) | 106 (34.8) | 82 (26.9) | 305 (100) |
a
Wild-type PomA and GFP-PomB (wild type and variants) were coexpressed in V. cholerae ΔpomAB. The total number of cells of each strain which were included in the analysis represents 100%.