Fig 3.

Characterization of purified PecS. (A) UV-visible absorption spectra of purified recombinant PecS. Insert, lanes 1 and 2, purified PecS in 10% native PAGE. (B) Purified recombinant PecS in a 15% SDS-PAGE gel. Lane 1, PecS; lane 2, molecular mass marker (Bio-Rad; molecular mass is indicated at the right). (C) Far-UV circular dichroism (CD) spectrum of purified PecS. Ellipticity measurements are represented in units of millidegrees (mdeg; machine units). (D) Melting temperature of PecS. Thermal denaturation is represented by the fluorescence emission resulting from the binding of SYPRO orange to denatured protein as a function of temperature.