Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant description | Source or referencea |
|---|---|---|
| Strains | ||
| E. coli DH5α | deoR endA1 gyrA96 hsdR17(rK− mK+) recA1 relA1 supE44 thi-1 Δ(lacZYA-argF)U169 ϕ80dlacZΔM15 F− | Bethesda Research Laboratories |
| P. multocida strains | ||
| AL523 | P. multocida VP161 strain containing Tn916 carrying tetM | This study |
| AL539 | VP161 insertional gatA mutant generated using pAL290; no expression of hptE due to polar effects of the gatA insertion | This study |
| AL806 | VP161 gatA mutant (AL539) containing pAL446 | |
| AL2116 | P1059 gatF (pm1141) TargeTron mutant of P1059 | This study |
| AL2117 | P1059 natB (pm1140) TargeTron mutant of P1059 | This study |
| AL2155 | P1059 gatG (pm1139) TargeTron mutant of P1059 | This study |
| AL2192 | P1059 gatG mutant containing pAL1022 | This study |
| AL2297 | P1059 gatF mutant containing pAL1046 | This study |
| AL2299 | P1059 natB mutant containing pAL1045 | This study |
| AL2354 | P1059 natB mutant containing vector pAL99T | This study |
| AL2355 | P1059 gatF mutant containing vector pAL99T | This study |
| AL2395 | P1059 gatG mutant containing vector pAL99S | This study |
| P1059 | Heddleston serovar 3 type strain | 32 |
| P1662 | Heddleston serovar 4 type strain | 32 |
| PM1 | P. multocida turkey isolate | 33 |
| PM3 | P. multocida turkey isolate | 33 |
| PM8 | P. multocida turkey isolate | 33 |
| PM18 | P. multocida chicken isolate | 33 |
| PM48 | P. multocida chicken isolate | 33 |
| PM72 | P. multocida chicken isolate | 33 |
| PM146 | P. multocida chicken isolate | 34 |
| PM1075 | P. multocida field isolate | MRG culture collection |
| PM1098 | P. multocida field isolate | MRG culture collection |
| PM1099 | P. multocida field isolate | MRG culture collection |
| PM1103 | P. multocida field isolate | MRG culture collection |
| PM1120 | P. multocida chicken isolate | MRG culture collection |
| PM1153 | P. multocida chicken isolate | MRG culture collection |
| PM1205 | P. multocida emu isolate | MRG culture collection |
| PM1258 | P. multocida chicken isolate | MRG culture collection |
| PM1268 | P. multocida chicken isolate | MRG culture collection |
| PM1320 | P. multocida chicken isolate | MRG culture collection |
| PM1369 | P. multocida chicken isolate | MRG culture collection |
| PM1434 | P. multocida chicken isolate | MRG culture collection |
| PM1439 | P. multocida chicken isolate | MRG culture collection |
| PM1441 | P. multocida turkey isolate | MRG culture collection |
| PM1470 | P. multocida turkey isolate | MRG culture collection |
| PM1474 | P. multocida duck isolate | MRG culture collection |
| Plasmids | ||
| pAL99 | P. multocida expression plasmid (Kanr) which contains the P. multocida constitutive Ptpi promoter | 35 |
| pAL99S | P. multocida expression plasmid (Specr); derivative of pAL99 | This study |
| pAL99T | P. multocida expression plasmid (Tetr); derivative of pAL99 | This study |
| pAL290 | pUA826 derivative containing an internal fragment of gatA | This study |
| pAL446 | Complete gctC and hptE genes from Pm70 cloned into pAL99 | This study |
| pAL692 | P. multocida plasmid (Specr) containing TargeTron group II intron | 20 |
| pAL953 | pAL692 with aph3 (Kanr) inserted into TargeTron group II intron | This study |
| pAL1003 | pAL953 retargeted to natB by using primers BAP6851/6852/6853 | This study |
| pAL1004 | pAL953 retargeted to gatG by using primers BAP6854/6855/6856 | This study |
| pAL1006 | pAL953 retargeted to gatF by using primers BAP6864/6865/6866 | This study |
| pAL1022 | Complete gatG gene cloned into pAL99S | This study |
| pAL1045 | Complete natB gene cloned into pAL99T | This study |
| pAL1046 | Complete gatF gene cloned into pAL99T | This study |
| pUA826 | Mob+ R6K replicon; single-crossover mutagenesis vector; Apr Spcr | 36 |
a
MRG, Microbiology Research Group, Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Australia.