Table 4.
Detected mutations in LPS outer core biosynthesis loci of P. multocida field isolates
| Longest LPS outer core structure detecteda | Predicted gene disrupted | Field isolate | Detected mutation(s)b |
|---|---|---|---|
| No detectable outer core | hptE | PM1075 | Q229* |
| PM1120 | Q194* | ||
| PM1258 | Single nucleotide deletion at position 61 | ||
| Hep·[Glc]·Glc | gatF | PM3 | None detected |
| PM8 | None detected | ||
| PM18 | A205T | ||
| PM146 | S41P, V138M | ||
| Hep·[Glc]·Glc·Gal | gatG | PM1098 | C128Y |
| PM1205 | Insertion of 42 nucleotides at position 353 | ||
| PM1268 | I50V, K98R, A253D, D254N, N259K, R272K, E277K, V279M, T284N, A288D, F297L | ||
| PM1320 | I50V, K98R, A253D, D254N, N259K, R272K, E277K, V279M, T284N, A288D, F297L | ||
| PM1439 | 14 nucleotides deleted, positions 496–509 | ||
| Hep·[Glc]·Glc·Gal·Gal | natB | PM1369 | None detected |
| PM1474 | None detected | ||
| Hep·[Glc]·Glc·Gal·Gal·GalNAc | natC | PM1 | Nucleotide changes/deletions in promoter region |
| PM48 | S97* | ||
| PM1099 | I132N, I206V | ||
| PM1103 | Deletion/rearrangement between nucleotides 186 and 276 | ||
| PM1441 | K3R, F4L, N7H, G33K, E49K, N62D, F78L, N80S, N105D, E115G, V120I, Y168D, K173N, Q176R, R193Q, I194V, Q207K, Q281K, I231V, E236K, E284D, K285Q, E271A, I292M, K296T, L312I, N396K, S397R | ||
| PM1434 | S97* | ||
| Hep·[Glc]·Glc·Gal·Gal·GalNAc·GalNAc | None | PM72 | All genes functional |
| PM1153 | All genes functional | ||
| PM1470 | All genes functional |
a
[Glc], residue is attached to the main chain residues via a 1,4 linkage to Hep.
b
Compared to nucleotide and amino acid sequences of the equivalent functional orthologues in Pm70 and/or P1059. *, stop codon. GatG residue changes shown in bold are in close proximity to amino acids predicted to interact with the donor or acceptor molecule or the divalent cation Mn2+.