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. 2013 Nov;195(21):4804–4815. doi: 10.1128/JB.00722-13

Table 1.

Bacterial strains and plasmids used in this study

Bacterial strain or plasmid Description or relevant characteristic(s) Source or reference
Bacterial strains
    MG1655 Wild-type E. coli K-12 D. Jin (NCI)
    DJ480 MG1655 ΔlacX74 D. Jin (NCI)
    CS104 DJ480 ΔsgrS 13
    CS123 DJ480 sgrS1 13
    CS168 DJ480 λattB::lacIq+ tetR Spr 23
    CS194 DJ480 ΔyigL::FRT λattB::lacIq+ tetR Spr This study
    CS195 DJ480 ΔyigL::FRT ΔsgrS λattB::lacIq+ tetR Spr This study
    JH111 DJ480 ΔsgrS λattB::lacIq+ tetR Spr 10
    JH116 DJ480 ΔsgrS manX′-′lacZ lacIq+ 10
    JH171 DJ480 ΔsgrS ptsG′-′lacZ lacIq+ 10
    YS185 DJ480 ΔyigL::FRT This study
    YS234 DJ480 ΔsgrS yigL′-′lacZ λattB::lacIq+ tetR Spr This study
    YS236 λattB::tet This study
    YS237 DJ480 λattB::tet This study
    YS238 DJ480 λattB::tet sgrS1 This study
    YS246 ΔsgrS::kan-araC-PBAD-ccdB; mini λ This study
    YS247 DJ480 λattB::tet ΔsgrS This study
    YS248 DJ480 sgrS28 This study
    YS249 DJ480 λattB::tet sgrS28 This study
    YS258 DJ480 ΔsgrS cm-Plac-ptsG λattB::lacIq+ tetR Spr This study
    YS259 DJ480 cm-Plac-ptsG λattB::lacIq+ tetR Spr This study
    YS265 DJ480 cm-Plac-ptsG yigL::FRT ΔsgrS λattB::lacIq+ tetR Spr This study
    YS269 DJ480 sgrS26 This study
    YS270 DJ480 λattB::tet sgrS26 This study
    YS273 DJ480 λattB::tet ΔyigL::FRT This study
    YS283 ΔmanXYZ::kan ΔsgrS λattB::tet This study
    YS284 ΔptsG::cm ΔsgrS λattB::tet This study
    YS285 ΔmanXYZ::kan λattB::tet This study
    YS286 ΔptsG::cm λattB::tet This study
Plasmids
    pBRCS12 Vector control for pLCV1, pBRCS6, pBRJH19, pBR26 and pBRYS4 13
    pLCV1 Plac-sgrS 5
    pBRCS6 Plac-sgrS1 13
    pBRJH26 Plac-sgrS26 10
    pBRYS4 Plac-sgrS28 This study
    pZE21 Vector control for pZEYS2 23
    pZEYS2 PLtetO-1-yigL This study