Fig 5.

BpuR does not bind bpuR promoter DNA. EMSAs of recombinant purified BpuR and bpuR 5′ noncoding DNA. (A) Labeled dsDNA (1 nM) corresponding to positions −142 to −71 relative to the bpuR translational start site; (B) labeled dsDNA (1 nM) corresponding to positions −70 to +1 relative to the bpuR translational start site; (C) labeled erp operator dsDNA (1 nM) serving as a positive control (19). Each probe was incubated either with 60 nM BpuR protein (lanes +) or without added BpuR (lanes −).