Figure 5.

Multiple UIM regions in ataxin-3 affect the same binding surface in the parkin Ubl domain. Regions of the ¹H–¹⁵N HSQC spectra for the ¹⁵N-labeled Ubl domain titrated with (A) UIM_123, (B) UIM_12Δ3, and (C) UIM_1Δ23. Identical residues that experienced chemical shift changes are labeled, and arrows indicate the direction of the shift with increasing concentrations of the different UIM constructs. The dotted arrow in spectrum C indicates that R75 experiences a chemical shift visible at a lower contour level. The concentrations of the ¹⁵N-labeled parkin Ubl domain were 105, 100, and 40 μM for UIM_123, UIM_12Δ3, and UIM_1Δ23 experiments, respectively.