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. 2013 Jul 30;19(21-22):2495–2505. doi: 10.1089/ten.tea.2012.0648

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 2. — Gene expression patterns of osteogenic markers in AdBMP2-transfected BMSCs following rhPDGF-BB treatment. The rhPDGF-BB treatment significantly suppressed the mRNA expression of (A) Runx2, (B) Dlx5, (C) Alp, and (D) Ibsp induced by the AdBMP2-transfected BMSCs, especially at 24 h. The mRNA expression of each osteogenic factor was normalized against Gapdh. *Statistically significant difference between AdBMP2-transfected BMSCs and AdBMP2-transfected BMSCs/rhPDGF-BB (ANOVA, p<0.05). ^#Statistically significant difference between nontransfected BMSCs and AdBMP2-transfected BMSCs (ANOVA, p<0.05). ^†Statistically significant difference between nontransfected BMSCs and AdBMP2-transfected BMSCs/rhPDGF-BB (ANOVA, p<0.05). Cell, nontransfected BMSCs; cells/B2, AdBMP2-transfected BMSCs; cells/B2P, AdBMP2-transfected BMSCs/rhPDGF-BB. Values represent mean±SD of three samples.