FIG. 3.
Gene expression patterns of BMP-2 related factors Bmpr1a (A), Bmpr2 (B), Noggin (C), and Gremlin (D) in AdBMP2-transfected BMSCs following the rhPDGF-BB treatment. The mRNA expression of both BMPRII and Noggin in the AdBMP2-transfected BMSCs was significantly decreased by the rhPDGF-BB treatment. The mRNA expression of each factor was normalized against Gapdh. *Statistically significant difference between AdBMP2-transfected BMSCs and AdBMP2-transfected BMSCs/rhPDGF-BB (ANOVA, p<0.05). #Statistically significant difference between nontransfected BMSCs and AdBMP2-transfected BMSCs (ANOVA, p<0.05). †Statistically significant difference between nontransfected BMSCs and AdBMP2-transfected BMSCs/rhPDGF-BB (ANOVA, p<0.05). Cell, nontransfected BMSCs; cells/B2, AdBMP2-transfected BMSCs; cells/B2P, AdBMP2-transfected BMSCs/rhPDGF-BB. Bmpr1a, BMP receptor type Ia; Bmpr2, BMP receptor type II. Values represent mean±SD of three samples.