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Urea concentrations in the conditioned medium of 2D (collagen-coated dishes) and 3D (liver organoid tissue) hepatocyte cultures. The relative amount of urea in the conditioned media after 72 h was calculated by setting the values obtained at 24 h to 100%. The absolute values (μg/106 cells) are noted in the corresponding columns. Mean±standard error (SE), n=3; *p<0.01.
