FIG. 8.
Transplanted liver organoid tissues in a partially hepatectomized mouse model. (A) A schematic illustration showing the transplantation of liver organoid tissue. Light micrograph (B) and fluorescent micrograph (C) images of transplanted DsRed2-expressing Hep G2 cells. (D–G) Micrographs of liver organoid tissue. (D) Fluorescent image. The red fluorescence indicates surviving DsRed-expressing Hep G2Red cells. Arrowheads indicate new blood vessels, which appear black. (E, F) Histological analyses: AZAN and hematoxylin–eosin staining, respectively, of liver organoid tissue sections after transplantation. Vascularization was detected at the condensed collagen fibril matrices. Arrowheads indicate new blood vessels. (G) Immunohistochemical analysis of the transplanted hepatic construct indicting albumin-positive hepatic cells and CD31/PECAM-1-positive endothelial cells using anti-albumin (red) and anti-CD31/PECAM-1 (green) antibodies. Arrowheads indicate new blood vessels. The scale bar corresponds to 200 μm. The animal transplantation experiments were carried out four times. Color images available online at www.liebertpub.com/tea