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. 2013 Oct 25;7:70. doi: 10.3389/fnsys.2013.00070

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Copyright © 2013 Sinakevitch, Smith, Locatelli, Huerta, Bazhenov and Smith.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Apis mellifera: The GABAergic processes in glomeruli express AmOA1 (A) Triple labeling of the anti-AmOA1 (green A1) and anti–GABA (magenta A2) in a glomerulus with neurobiotin-injected uPNs (blue A3). (A3) The fibers that enter into the glomerulus are GABAergic co-stained with AmOA1 (white, arrow), non-GABAergic processes co-stained with AmOA1 (green, arrowhead) and uPNs that do not co-localize with AmOA1 (blue, double arrowheads). (B) GABA and AmOA1 staining at higher magnification (C) Triple labeling of a glomerulus (C1) in which anti-AmOA1 (green, C1 and C2 single image) and ORNs were labeled by anterograde staining with Rhodamine-dextran (magenta C1, single image C3), and uPNs are shown by retrograde staining (blue C1, and black and white in a single image C4). (D) The same glomerulus as in (C1) but only ORNs (magenta) and AmOA1(green) are shown (E) illustrates a high magnification of the area shown in the square designating in (D). Arrows show the close proximity of the AmOA1 stained profiles and ORNs. (F) The same glomerulus as in (C1) but only staining of AmOA1 and uPNs are shown. (G) A high magnification of the area shown by the square designated in (F). Arrowheads show AmOA1 in the area that surrounds the uPNs fibers, which might be presynaptic to uPNs. (H) Detail of the antennal lobe labeled with anti-AmOA1 (green, H1) and anterogradely labeled ORNs (magenta H2 in merge image). ORNs do not show the co-expression with AmOA1. (H3) PNs are labeled by injection into the LPL where three glomeruli from the lateral part of the antennal lobe are shown with cell bodies surrounding the antennal lobe. (H4) Merged image where anti-AmOA1 (green) and PNs (magenta) illustrate that there are possible co-localizations of AmOA1 in the cell bodies of subsets of PNs. Scale bars: A,C,D,F = 15 μm; B,E,G = 5 μm; H = 35 μm.