Figure 2.

Promoter methylation of CYP24A1 in adjacent mucosa and tumor tissue. Schematic overview of the CYP24A1 promoter region including position of regulatory elements (vitamin D responsive elements (VDRE1 and VDRE2), specificity protein 1 (SP1), vitamin D stimulatory element (VSE) and TATA box) as well as position of bisulfite genomic sequencing primers (black arrows) are shown (a). Bisulfite genomic sequencing of the CYP24A1 promoter was performed in 20 human adenocarcinoma and 20 adjacent mucosa samples. For each region, the mean methylation per sample is blotted, median is indicated (b). Incidence of CpG nucleotide methylation is shown for tumor and adjacent mucosa (c).