Table 1.
Primer | Accession Number | Role | Primers (5′→3′) | Coordinates |
---|---|---|---|---|
GAPDH | NM_002046.3 | Forward | CTTCTTTTGCGTCGCCAGCCGA | 62–83 |
Reverse | CACGACGTACTCAGCGCCAGC | 390–370 | ||
CYP1A1 | NM_000499.3 | Forward | GTACCTCAGCCACCTCCAAGAT | 88–109 |
Reverse | GCTCCTGCAACGTGCTTATC | 632–651 | ||
CYP1A2 | NM_000761.3 | Forward | CATCCCCCACAGCACAACAAGGG | 1219–1241 |
Reverse | CAGTTGATGGAGAAGCGCAGCC | 1593–1614 | ||
CYP2B6 | NM_000767.4 | Forward | GTCGACCCATTCTTCCGGGGAT | 317–338 |
Reverse | GAAGAGCTCAAACAGCTGGCCGAA | 644–667 | ||
CYP2C19 | NM_000769.1 | Forward | TGGAGAAGGAAAAGCAAAACCAAC | 812–835 |
Reverse | GGTTGTGCCCTTGGGAATGAGG | 1137–1158 | ||
CYP2E1 | NM_000773.3 | Forward | ACCCAAGGCCAGCCTTTCGAC | 514–534 |
Reverse | TCCACGAGCAGGCAGTCGGT | 829–848 | ||
CYP3A4 | NM_017460.5 | Forward | GGAAAAGTGTGGGGCTTTTATGATGG | 307–333 |
Reverse | GCCTGTCTCTGCTTCCCGCC | 589–608 | ||
CYP3A5 | NM_000777.3 | Forward | TTCACCATGACCCAAAGTACTGGAC | 1304–1321 |
Reverse | TAACTCATTCTCCACTTAGGGTTCC | 1591–1615 | ||
PXR | NM_022002.2 | Forward | CAAGCGGAAGAAAAGTGAACG | 540–560 |
Reverse | CTGGTCCTCGATGGGCAAGTC | 961–981 |