Figure 3.

CRK-SH3N domain expression reduces the motility of NSCLC cells. (A) Standard would healing assay in H157 and A549 NSCLC cells stably expressing pCMV (empty vector), CRK-SH2 domain, or CRK-SH3N domain. (B) Quantification of the wound area among cells expressing pCMV, CRK-SH2 domain, or CRK-SH3N domain at 48 hours. Experiments were done in triplicate. Error bars represent ± standard deviation. Comparison of the wound surface area means between the groups was performed by a 2-sided Student’s t test. NS = nonsignificant (P > 0.05); ***P < 0.0001.