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. 2013 Oct 1;382(1):15–26. doi: 10.1016/j.ydbio.2013.08.004

Fig. 1.

Fig. 1

Blimp1 is expressed by dermal papilla cells during skin morphogenesis. (A–D) Cryosections of E13.5-P2 skin labelled with antibodies to Sox2 and Blimp1. Note Blimp1 expression in DP of guard/awl/auchene hair follicles (red arrows) and zigzag hair follicles (green arrows) and in the outermost epidermal cell layers (B–D). (E–I) GFP expression under the control of Blimp1 regulatory elements colocalizes with endogenous Blimp1 during morphogenesis of guard/awl/auchene (E–G) and zigzag (H–I) hairs. Arrows show dermal condensates (F, G) and dermal papilla (H). (J) Blimp1 mRNA was detected in guard/awl/auchene (CD133+Sox2GFP+) and zigzag (CD133+Sox2GFP−) dermal papilla cells, but not in dermal fibroblasts (CD133−Sox2GFP−). (K, L) Flow cytometry of single cell suspensions of E18.5 (H) and P2 (I) dermal cells, showing co-expression of the dermal papilla marker CD133 with Blimp1GFP. Cells in each quadrant are labelled with a different colour for ease of visualisation. Data are representative of at least N=3 biological replicates. Scale bars: (A, B) 1 mm, (C–G) 100 μm.