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. 2013 Oct 1;382(1):15–26. doi: 10.1016/j.ydbio.2013.08.004

Fig. 9.

Fig. 9

Touch dome innervation on Sox2 deletion. (A) E18.5 K14CreSox2fl/- back skin wholemount immunostained for K8 and PGP9.5 and visualised from the epidermal side, showing PGP9.5+ nerves interacting with touch domes. (B, C) E18.5 back skin wholemounts from K14CreSox2fl/− and K14CreSox2fl/fl mice immunostained with PGP9.5 to illustrate nerve density. (D) Quantification of nerve density in K14CreSox2fl/− and K14CreSox2fl/fl mice (n=8 biological replicates). (E) Single plane confocal image of horizontal wholemount from K14CreSox2fl/− E18.5 back skin immunostained with PGP9.5 and K8. Arrows indicate nerve–Merkel cell interaction; encircled Merkel cells are not associated with nerves. (F, G) Confocal z-stacks showing touch dome innervation in K14CreSox2fl/− and K14CreSox2fl/fl mouse back skin. Horizontal wholemounts were immunostained for PGP9.5 and K8. (H) Quantification of % of innervated Merkel cells per touch dome in K14CreSox2fl/− and K14CreSox2fl/fl mice (n=4 biological replicates). Scale bars: 100 μm (A–C); 25 μm (E–G).