Table 1.

Summary of hot spot prediction methods.

Hotspot prediction	Properties Used	Availability	Technique	Reference
FoldX	Energy-based	Tool & Server	Computational alanine scanning	[70]
Robetta	Energy-based	Server	Computational alanine scanning	[71]
PP_Site	Energy, structure-based	Tool	Simple algorithm	[72]
FTMAP	Energy –based	Tool & Server	Probe based rigid body docking with fast Fourier transform correlation	[73]
PCRPi, PCRPi-W	Energy, structure, evolutionary	Tool & Server	Bayesian Network	[74,75]
Guhary & Chakrabarti	H-bonding, interface location	Tool	Simple algorithm	[76]
KFC2	ASA, various structural features	Server	Support Vector Machine (SVM)	[77]
MINERVA	Structure, sequence, molecular interaction	Tool	Descision Tree, SVM	[78]
HSPred	Energy-based	Tool	SVM (Residue specific)	[79, 80]
Rajamani	Side chain ΔASA	Tool	Molecular Dynamics	[59]
Higa & Tozzi	Structural, evolutionary	Tool	SVM	[81]
Grosdidier & Recio	Energy-based (Docking)	Tool	Normalized interface propensity	[82]
HotPoint HotSprint	Conservation, accessibility, residue propensity	Server & Database	Empirical formula	[83, 84]