Figure 4.

Ion Mobility Measurement of the CSM Complex and Its Subcomplexes

(A) CCS values measured for the intact complex (purple circle), the 357 kDa (green diamond) and 216 kDa (orange square) subcomplexes, and the largest subunit Sso1428 (red triangle) are plotted against their masses. Three trendlines are shown for linear, linear dimer, or collapsed “globular” conformations (left to right) for complexes composed of monomers (25 kDa). Considerable deviation from all conformation is evident for the intact complex and the two subcomplexes.

(B–D) Coarse-grain structural models, calculated for the intact complexes (D) and the 357 kDa (C) and 216 kDa (B) subcomplexes and fitted into the CSM EM map. Each subunit is represented by a sphere, sized proportionally to its mass, except that the largest Sso1428 is divided into two domains.