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Five primary clusters making up a single secondary collecting duct (CD) cluster that consists of 31 CDs (aquaporin- 2, blue) at the inner medulla (IM) base. Image taken from section at 400 μm below IM base. CD cluster boundaries (white) determined by Euclidean distance map technique. Polygons (red) delimit the intracluster interstitium for each of the five primary CD clusters. Scalebar, 100 μm. From Pannabecker et al. (2008b), used with permission.
