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Five primary clusters making up a single secondary collecting duct (CD) cluster that consists of 31 CDs at the inner medulla (IM) base. Image taken from section at 400 μm below IM base. CD cluster boundaries (white) determined by Euclidean distance map technique. Descending thin limb/aquaporin-1 (magenta), CD/aquaporin-2 (blue), ascending thin limb/chloride channel ClC-K1 (green). Scalebar, 100 μm. From Pannabecker et al. (2008b), used with permission.
