Figure S4.
Antimicrobial Peptide Expression in Flies Expressing Mef2-EnR or Receiving Separate M. luteus and E. coli Infections, Related to Figure 4
(A) Induction of Diptericin and Metchnikowin are impaired by expression of a Mef2-engrailed repressor fusion protein. Mef2-EnR-expressing (w, UAS-Mef2-EnR / Y ; tub-Gal80ts / + ; tub-Gal4 / +) and control (w, UAS-Mef2-EnR / Y ; + ; +) flies were maintained at 18° until three days before infection, when they were shifted to 29°. Samples collected 3 and 6 hr after infection (p.i.).Values are mean ± standard deviation. Significance levels: ∗p < 0.01; ∗∗p < 0.001, by heteroscedastic unpaired two-tailed t test.
(B) Expression of four antimicrobial peptides in Mef2-knockdown and control flies after infection with E. coli (Gram-negative, imd agonist) or M luteus (Gram-positive, Toll agonist), as assayed by qRT-PCR, normalized to uninjected controls. Samples collected 3 and 6 hr after infection (p.i.). Values are mean ± standard deviation. Significance levels: ∗p < 0.01; ∗∗p < 0.001, by heteroscedastic unpaired two-tailed t test.