Table 2.

Primers for RT-PCR Analysis of MC3T3-E1 Cells

Gene	Forward primer	Reverse primer
GAPDH	AACGACCCCTTCATTGAC	TCCACGACATACTCAGCAC
OPN	GATCAGGACAACAACGGAAAGG	CTTGTGGCTGTGAAACTTGTGG
OCN	AGGGAGGATCAAGTCCCG	GAACAGACTCCGGCGCTA
ALP	GTTGCCAAGCTGGGAAGAACAC	CCCACCCCGCTATTCCAAAC
BSP	TGTCTGCTGAAACCCGTTC	GGGGTCTTTAAGTACCGGC
OPG	CGAGGACCACAATGAACAAGTG	TTTTAGGTAGGTGCCAGGAGCA

ALP, alkaline phosphatase; BSP, bone sialoprotein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; OCN, osteocalcin; OPG, osteoprotegerin; OPN, osteopontin.