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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Methods. 2012 Oct 24;62(1):10.1016/j.ymeth.2012.09.016. doi: 10.1016/j.ymeth.2012.09.016

Figure 2. Finding the periphery.

Figure 2

(A) The embryo image is divided into 60 domains radiating out from the image center (yellow lines). The two cyan lines represent the boundaries of the domain shown in (B).

(B) Intensity of the pixels in the domain shown in (A) as a function of radial distance from the center of the image. The red circle denotes the outermost point in which the image intensity rapidly drops from high to low, signifying the periphery of the embryo in this domain.

(C) For each domain shown in (A), the points where the boundary of the embryo is determined to be.